Part:BBa_K5226014

Vib-gcvP

Introduction

One of the goals of iGEM24-SCUT-China-A is to use synthetic biology tools to obtain Halomonas TD strains that can metabolize formate. We chose to introduce the formate assimilation pathway to enable it to utilize formate, a difficult-to-recover product in CDE. As a second approach, based on the homology between Vibrio natriegens and Halomonas TD, we chose to import the C1, C2, and C3 modules from Vibrio natriegens (parts:).

Usage and Biology

Vib-GcvP is glycine dehydrogenase. The protein sequence is fromVibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759. This protein acts in conjunction with GvcH to form H-protein-S-aminomethyldihydrolipoyllysine from glycine during the glycine cleavage system(C2M).This part is used in BBa_xx and BBa_xx.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 546
  Illegal XbaI site found at 1602
  Illegal PstI site found at 2237
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1629
  Illegal NheI site found at 2226
  Illegal PstI site found at 2237
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 353
  Illegal BglII site found at 1638
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 546
  Illegal XbaI site found at 1602
  Illegal PstI site found at 2237
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 546
  Illegal XbaI site found at 1602
  Illegal PstI site found at 2237
  
• 1000
  COMPATIBLE WITH RFC[1000]